Hotspot

rpl32-trnL-UAG

SSC · 117368-118490 · 1123 bp

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Pi 0.0208
MarkerSeek score 71.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1123
variable_sites
35
pi
0.0208
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTAAAAGCGTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1234-1259 bp 1.0 85.6
rpl32-trnL-UAG_p2 GGGCAGCATTAAAAGCGTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1235-1260 bp 1.0 85.5
rpl32-trnL-UAG_p3 GGGCAGCATTAAAAGCGTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 1244-1269 bp 1.0 84.6
rpl32-trnL-UAG_p4 GGGCAGCATTAAAAGCGTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.9 57.8 1243-1268 bp 1.0 84.0
rpl32-trnL-UAG_p5 GGGCAGCATTAAAAGCGTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.9 57.8 1242-1267 bp 1.0 84.0