Hotspot

trnP-UGG-psaJ

LSC · 69260-69848 · 589 bp

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Pi 0.0385
MarkerSeek score 63.70
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
589
variable_sites
34
pi
0.0385
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 679-685 bp 1.0 86.9
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 678-684 bp 1.0 86.9
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 690-696 bp 1.0 86.8
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 689-695 bp 1.0 86.8
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 692-698 bp 1.0 86.7