Hotspot

psbF-psbE

LSC · 67172-67180 · 9 bp

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Pi 0.0741

MarkerSeek score 66.20

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GAACAGCCAACCATCGTACT	AACTGGCCGTTTTGATCCTT	50.0	45.0	57.9	58.0	116 bp	1.0	79.9
psbF-psbE_p2	GAACAGCCAACCATCGTACT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	115 bp	1.0	79.9
psbF-psbE_p3	GAACAGCCAACCATCGTACT	GAGCCGACAAGGAATTCCAT	50.0	50.0	57.9	58.0	140 bp	1.0	79.5
psbF-psbE_p4	GAACAGCCAACCATCGTACT	TACAGAGAGCCGACAAGGAA	50.0	50.0	57.9	58.1	146 bp	1.0	79.1
psbF-psbE_p5	GAACAGCCAACCATCGTACT	TTACAGAGAGCCGACAAGGA	50.0	50.0	57.9	58.1	147 bp	1.0	79.1