Hotspot

rbcL-accD

LSC · 59103-60051 · 949 bp

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Pi 0.0183

MarkerSeek score 44.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 26
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTCGAAGCAATGGATACTTT	CCCCGGATAAACAACATCGA	35.0	50.0	52.9	57.7	1014-1184 bp	1.0	42.0
rbcL-accD_p2	ATTCGAAGCAATGGATACTT	CCCCGGATAAACAACATCGA	35.0	50.0	52.6	57.7	1015-1185 bp	1.0	42.0
rbcL-accD_p3	ATTCGAAGCAATGGATACTTT	CCCCGGATAAACAACATCGA	33.3	50.0	53.6	57.7	1015-1185 bp	1.0	42.0
rbcL-accD_p4	TTCGAAGCAATGGATACTTT	CCCCGGATAAACAACATCGAA	35.0	47.6	52.9	58.4	1014-1184 bp	1.0	42.0
rbcL-accD_p5	ATTCGAAGCAATGGATACTT	CCCCGGATAAACAACATCGAA	35.0	47.6	52.6	58.4	1015-1185 bp	1.0	42.0