Hotspot

ndhC-trnV-UAC

LSC · 53151-53823 · 673 bp

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Pi 0.0594
MarkerSeek score 83.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
673
variable_sites
60
pi
0.0594
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 823-1096 bp 1.0 79.9
ndhC-trnV-UAC_p2 TCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 824-1097 bp 1.0 79.8
ndhC-trnV-UAC_p3 CCCTTTGCTAATCGGAGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.1 57.8 817-1090 bp 1.0 78.3
ndhC-trnV-UAC_p4 TTTCTCTGGCCCTTTGCTAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.0 57.8 826-1099 bp 1.0 77.7
ndhC-trnV-UAC_p5 TTCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 47.6 50.0 58.0 57.8 825-1098 bp 1.0 77.5