Hotspot

trnG-GCC-trnfM-CAU

LSC · 38837-39115 · 279 bp

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Pi 0.0311

MarkerSeek score 64.50

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 13
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	326-357 bp	1.0	85.3
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	324-355 bp	1.0	84.2
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	347-378 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	346-377 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	GAGGTCACGGGTTCAAATCC	50.0	55.0	57.8	58.5	323-354 bp	1.0	81.3