Hotspot

psbZ-trnG-GCC

LSC · 38210-38765 · 556 bp

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Pi 0.03
MarkerSeek score 60.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
556
variable_sites
25
pi
0.03
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TGGATTAGTCTTTCTAGTGGGT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 55.9 57.8 600-649 bp 1.0 53.1
psbZ-trnG-GCC_p2 TTGGATTAGTCTTTCTAGTGGGT CGTCTTCTCCTTGGCAAAGA 39.1 50.0 56.6 57.8 601-650 bp 1.0 50.9
psbZ-trnG-GCC_p3 TCTGGTACATCATTATGGATTGGA CGTCTTCTCCTTGGCAAAGA 37.5 50.0 57.2 57.8 620-669 bp 1.0 47.6
psbZ-trnG-GCC_p4 ATTGGATTAGTCTTTCTAGTGGGT CGTCTTCTCCTTGGCAAAGA 37.5 50.0 57.1 57.8 602-651 bp 1.0 47.2
psbZ-trnG-GCC_p5 TGGATTAGTCTTTCTAGTGGG CGTCTTCTCCTTGGCAAAGA 42.9 50.0 54.1 57.8 600-649 bp 1.0 47.2