Hotspot

trnR-UCU-atpA

LSC · 10270-10982 · 713 bp

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Pi 0.0374
MarkerSeek score 64.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
713
variable_sites
40
pi
0.0374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TCAGGAACAGATGGAACTCT 47.6 45.0 57.3 55.5 787-891 bp 1.0 54.1
trnR-UCU-atpA_p2 GGTTCAAATCCTATTGGACGC TCAGGAACAGATGGAACTCTT 47.6 42.9 57.3 56.3 787-891 bp 1.0 52.6
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC TTCAGGAACAGATGGAACTCT 47.6 42.9 57.3 56.3 788-892 bp 1.0 52.6
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC AGCTATTCAGGAACAGATGGA 47.6 42.9 57.3 56.1 793-897 bp 1.0 51.4
trnR-UCU-atpA_p5 GGTTCAAATCCTATTGGACGC TCAGGAACAGATGGAACTCTTT 47.6 40.9 57.3 57.0 787-891 bp 1.0 50.5