Hotspot

rps16-trnQ-UUG

LSC · 6168-6744 · 577 bp

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Pi 0.03
MarkerSeek score 59.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
577
variable_sites
26
pi
0.03
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 645-671 bp 1.0 85.0
rps16-trnQ-UUG_p2 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 649-675 bp 1.0 83.5
rps16-trnQ-UUG_p3 CAAGTCGCACGTTGCTTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.5 58.1 651-677 bp 1.0 82.9
rps16-trnQ-UUG_p4 GTCGCACGTTGCTTTTTACC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 648-674 bp 1.0 81.7
rps16-trnQ-UUG_p5 GCACGTTGCTTTTTACCACA TTCGGAGGTTCGAATCCTTC 45.0 50.0 57.8 57.3 649-675 bp 1.0 80.3