Hotspot

trnK-UUU-rps16

LSC · 4352-5044 · 693 bp

Back to Dialium

Pi 0.025

MarkerSeek score 64.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 26
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	GCGGGTGTTTCTATGGAACT	50.0	50.0	58.6	57.9	789-1352 bp	1.0	77.7
trnK-UUU-rps16_p2	GAGTCGCACTTAAAAGCCGA	GCGGGTGTTTCTATGGAACT	50.0	50.0	58.6	57.9	790-1353 bp	1.0	77.6
trnK-UUU-rps16_p3	TCGAGTCGCACTTAAAAGCC	GCGGGTGTTTCTATGGAACT	50.0	50.0	58.6	57.9	792-1355 bp	1.0	77.6
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	AAAGGGCGGGTGTTTCTATG	50.0	50.0	58.6	58.2	794-1357 bp	1.0	77.2
trnK-UUU-rps16_p5	GAGTCGCACTTAAAAGCCGA	AAAGGGCGGGTGTTTCTATG	50.0	50.0	58.6	58.2	795-1358 bp	1.0	77.2