Hotspot

ycf2

IRb · 90735-97634 · 6900 bp

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Pi 0.00705

MarkerSeek score 45.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6900
variable_sites: 95
pi: 0.00705
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GATCGGTCGCTCAAACTCTT	ACAGAGACAGGGGACTCATC	50.0	55.0	58.0	58.1	829-835 bp	1.0	82.1
ycf2_p2	GATCGGTCGCTCAAACTCTT	GACAGGGGACTCATCTCTGA	50.0	55.0	58.0	57.8	824-830 bp	1.0	82.1
ycf2_p3	GATCGGTCGCTCAAACTCTT	AGACAGGGGACTCATCTCTG	50.0	55.0	58.0	57.8	825-831 bp	1.0	82.0
ycf2_p4	GATCGGTCGCTCAAACTCTT	CAGAGACAGGGGACTCATCT	50.0	55.0	58.0	57.8	828-834 bp	1.0	82.0
ycf2_p5	ACAGTATGCGCTTCAAGAGG	ACAGAGACAGGGGACTCATC	50.0	55.0	58.0	58.1	267 bp	0.4	60.1