Hotspot

clpP

LSC · 75426-75794 · 369 bp

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Pi 0.0396

MarkerSeek score 74.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 31
pi: 0.0396
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CGGATTCAAAGTGCCATGCT	GAAGAAGCGACTTGGGTTGA	50.0	50.0	59.2	58.1	430-436 bp	1.0	76.4
clpP_p2	TCGGATTCAAAGTGCCATGC	GAAGAAGCGACTTGGGTTGA	50.0	50.0	59.2	58.1	431-437 bp	1.0	76.4
clpP_p3	CGGATTCAAAGTGCCATGC	GAAGAAGCGACTTGGGTTGA	52.6	50.0	57.7	58.1	430-436 bp	1.0	75.2
clpP_p4	CGGATTCAAAGTGCCATGCT	TGGTGTTCCAAAACTAGCGT	50.0	45.0	59.2	57.7	467-473 bp	1.0	74.1
clpP_p5	TCGGATTCAAAGTGCCATGC	TGGTGTTCCAAAACTAGCGT	50.0	45.0	59.2	57.7	468-474 bp	1.0	74.1