Hotspot

trnL-CAA-ycf2

IRa · 148384-149601 · 1218 bp

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Pi 0.0149

MarkerSeek score 56.30

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 1218
variable_sites: 34
pi: 0.0149
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	GATCGGTCGCTCAAACTCTT	50.0	50.0	58.0	58.0	1982-2021 bp	1.0	83.3
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	GATCGGTCGCTCAAACTCTT	50.0	50.0	58.0	58.0	1984-2023 bp	1.0	83.3
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	TTTTGAGTTGTTCTGCCGGA	50.0	45.0	58.0	58.0	2000-2039 bp	1.0	82.9
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	ACAGTATGCGCTTCAAGAGG	50.0	50.0	58.0	58.0	1420-1448 bp	0.4	57.4
trnL-CAA-ycf2_p5	TGAAATGGTAGACACGCGAG	ACAGTATGCGCTTCAAGAGG	50.0	50.0	58.0	58.0	1422-1450 bp	0.4	57.4