Hotspot

trnR-ACG-rrn5

IRa · 134137-134387 · 251 bp

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Pi 0.093

MarkerSeek score 90.30

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: IRa
length_bp: 251
variable_sites: 44
pi: 0.093
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 90.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	CGTCGAAGCGAAAATAACCG	CCTTGTTCCTACATGACCCG	50.0	55.0	57.9	58.0	1084 bp	0.2	47.4
trnR-ACG-rrn5_p2	ACACCGTCGAAGCGAAAATA	CCTTGTTCCTACATGACCCG	45.0	55.0	57.9	58.0	1088 bp	0.2	47.3
trnR-ACG-rrn5_p3	CGTCGAAGCGAAAATAACCG	CCTCGCAATCACTAGCCAAT	50.0	50.0	57.9	58.0	917 bp	0.2	47.2
trnR-ACG-rrn5_p4	CGTCGAAGCGAAAATAACCG	TGTCCCCTCCAGTCAAGAAT	50.0	50.0	57.9	58.0	942 bp	0.2	47.2
trnR-ACG-rrn5_p5	CGTCGAAGCGAAAATAACCG	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	57.9	58.0	1145 bp	0.2	47.2