Hotspot

trnN-GUU-trnG-UCC

IRa · 133332-133946 · 615 bp

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Pi 0.0375

MarkerSeek score 75.20

Primer available yes

Label trnN-GUU-trnG-UCC

Feature Metadata

label_name: trnN-GUU-trnG-UCC
feature_type: igs
region: IRa
length_bp: 615
variable_sites: 42
pi: 0.0375
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnG-UCC_p1	AGAAGTTCCGGGAGTTACGA	CCTCGCAATCACTAGCCAAT	50.0	50.0	58.1	58.0	1394-1412 bp	1.0	88.1
trnN-GUU-trnG-UCC_p2	TGGGTTGAGAACGGGAATTG	CCTCGCAATCACTAGCCAAT	50.0	50.0	58.1	58.0	1348-1366 bp	1.0	88.1
trnN-GUU-trnG-UCC_p3	AGAAGTTCCGGGAGTTACGA	TGTCCCCTCCAGTCAAGAAT	50.0	50.0	58.1	58.0	1419-1437 bp	0.8	76.5
trnN-GUU-trnG-UCC_p4	TAAGGATACGATAGCGGCCA	CCTCGCAATCACTAGCCAAT	50.0	50.0	58.1	58.0	1796-1812 bp	0.6	67.6
trnN-GUU-trnG-UCC_p5	TAAGGATACGATAGCGGCCA	TGTCCCCTCCAGTCAAGAAT	50.0	50.0	58.1	58.0	1821-1837 bp	0.6	67.5