Hotspot

rpl32-trnL-UAG

SSC · 117269-118052 · 784 bp

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Pi 0.0276
MarkerSeek score 65.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
784
variable_sites
46
pi
0.0276
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAGGATTGGCGGAATTCAC GGAGAGCGGGGACTAATAGT 50.0 55.0 58.0 58.0 1855-1873 bp 0.8 74.8
rpl32-trnL-UAG_p2 AGGATTGGCGGAATTCACTC GGAGAGCGGGGACTAATAGT 50.0 55.0 58.0 58.0 1853-1871 bp 0.8 74.8
rpl32-trnL-UAG_p3 GAGGATTGGCGGAATTCACT GGAGAGCGGGGACTAATAGT 50.0 55.0 58.0 58.0 1854-1872 bp 0.8 74.8
rpl32-trnL-UAG_p4 GGATTGGCGGAATTCACTCT GGAGAGCGGGGACTAATAGT 50.0 55.0 58.0 58.0 1852-1870 bp 0.8 74.8
rpl32-trnL-UAG_p5 GGATTGGCGGAATTCACTCT TGGAGAGCGGGGACTAATAG 50.0 55.0 58.0 58.0 1853-1871 bp 0.8 74.8