Hotspot

trnN-GUU-ndhF

SSC · 113686-114720 · 1035 bp

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Pi 0.0422

MarkerSeek score 82.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 1035
variable_sites: 91
pi: 0.0422
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GGAAGGGTATTATCCACGCA	50.0	50.0	58.2	57.1	1126-1137 bp	1.0	80.9
trnN-GUU-ndhF_p2	GGATTCGAACCTACGACCAG	GGAAGGGTATTATCCACGCA	55.0	50.0	57.8	57.1	1125-1136 bp	1.0	80.5
trnN-GUU-ndhF_p3	CCCCAAGTAGGATTCGAACC	GGAAGGGTATTATCCACGCA	55.0	50.0	57.7	57.1	1134-1145 bp	1.0	79.5
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	AGGAAGGGTATTATCCACGC	50.0	50.0	58.2	56.8	1127-1138 bp	1.0	78.5
trnN-GUU-ndhF_p5	GGATTCGAACCTACGACCAG	AGGAAGGGTATTATCCACGC	55.0	50.0	57.8	56.8	1126-1137 bp	1.0	78.1