Hotspot

trnR-ACG-trnN-GUU

IRb · 112883-113613 · 731 bp

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Pi 0.0213

MarkerSeek score 70.10

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 731
variable_sites: 31
pi: 0.0213
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CCTCGCAATCACTAGCCAAT	AGAAGTTCCGGGAGTTACGA	50.0	50.0	58.0	58.1	1394-1412 bp	1.0	87.0
trnR-ACG-trnN-GUU_p2	CCTTGTTCCTACATGACCCG	AGAAGTTCCGGGAGTTACGA	55.0	50.0	58.0	58.1	1561-1579 bp	1.0	86.8
trnR-ACG-trnN-GUU_p3	CCTTGTTCCTACATGACCCG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.0	58.1	1515-1533 bp	1.0	86.8
trnR-ACG-trnN-GUU_p4	GTCAAGTGGAAGTGCAGTGA	AGAAGTTCCGGGAGTTACGA	50.0	50.0	58.0	58.1	1622-1640 bp	1.0	86.5
trnR-ACG-trnN-GUU_p5	TGTCCCCTCCAGTCAAGAAT	AGAAGTTCCGGGAGTTACGA	50.0	50.0	58.0	58.1	1419-1437 bp	0.8	77.8