Hotspot

trnH-GUG-ycf2

IRb · 90517-90734 · 218 bp

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Pi 0.0615

MarkerSeek score 74.10

Primer available yes

Label trnH-GUG-ycf2

Feature Metadata

label_name: trnH-GUG-ycf2
feature_type: igs
region: IRb
length_bp: 218
variable_sites: 26
pi: 0.0615
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-ycf2_p1	GTTGGGCGCTTTAACCATTC	ACTGAATTGAATTGGGTCCATG	50.0	40.9	58.0	56.9	353-355 bp	1.0	61.4
trnH-GUG-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCCTACTGAATTGAATTGGGTCC	50.0	43.5	58.0	58.1	357-359 bp	1.0	60.8
trnH-GUG-ycf2_p3	ATTGGCATGCATCCAGTAGG	ACTGAATTGAATTGGGTCCATG	50.0	40.9	58.0	56.9	402-404 bp	1.0	60.7
trnH-GUG-ycf2_p4	ATTGGCATGCATCCAGTAGG	TCCTACTGAATTGAATTGGGTCC	50.0	43.5	58.0	58.1	406-408 bp	1.0	60.1
trnH-GUG-ycf2_p5	ATTGGCATGCATCCAGTAGG	ACTGAATTGAATTGGGTCCA	50.0	40.0	58.0	54.8	402-404 bp	1.0	59.8