Hotspot

petA-psbJ

LSC · 67380-68400 · 1021 bp

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Pi 0.0137

MarkerSeek score 56.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1021
variable_sites: 28
pi: 0.0137
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GGGTCATATTCCGGATTGGG	50.0	55.0	57.8	57.8	1144-1158 bp	1.0	81.3
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1137-1151 bp	1.0	80.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGGTCATATTCCGGATTGGG	50.0	55.0	58.7	57.8	1151-1165 bp	1.0	77.1
petA-psbJ_p4	GATCCATTACGCGTCCAAGG	GGGTCATATTCCGGATTGGG	55.0	55.0	58.8	57.8	1162-1176 bp	1.0	76.2
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.7	57.6	1144-1158 bp	1.0	75.8