Hotspot

ndhC-trnV-UAC

LSC · 52904-53979 · 1076 bp

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Pi 0.022

MarkerSeek score 74.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 44
pi: 0.022
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1178-1208 bp	1.0	83.5
ndhC-trnV-UAC_p2	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1180-1210 bp	1.0	81.7
ndhC-trnV-UAC_p3	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1181-1211 bp	1.0	81.7
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCAGAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.8	57.5	1177-1207 bp	1.0	80.8
ndhC-trnV-UAC_p5	TTCCGGCCCTTTACTAATCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	1196-1226 bp	1.0	80.1