Hotspot

psaA-ycf3

LSC · 44101-44895 · 795 bp

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Pi 0.0227
MarkerSeek score 77.70
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
795
variable_sites
40
pi
0.0227
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCAGGTTTAGCCCATTCCTC ATCAAGCCGCTGAGTATTGG 55.0 50.0 57.9 58.0 976-991 bp 1.0 83.9
psaA-ycf3_p2 CCAGGTTTAGCCCATTCCTC GGAAACAGGCTATAGCGCTT 55.0 50.0 57.9 58.0 958-973 bp 1.0 83.9
psaA-ycf3_p3 CCAGGTTTAGCCCATTCCTC TTGGTTGAAGATCACCAGGC 55.0 50.0 57.9 58.1 909-924 bp 1.0 83.8
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 949-964 bp 1.0 83.5
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 967-982 bp 1.0 83.4