Hotspot

ndhF-rpl32

SSC · 114483-115633 · 1151 bp

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Pi 0.00564

MarkerSeek score 55.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1151
variable_sites: 30
pi: 0.00564
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	GCTGCCCAATATCCCTTCTT	50.0	50.0	58.0	57.6	1331-1356 bp	1.0	80.4
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	GCTGCCCAATATCCCTTCTT	45.0	50.0	58.0	57.6	1329-1354 bp	1.0	80.3
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	GCTGCCCAATATCCCTTCTT	45.0	50.0	58.0	57.6	1330-1355 bp	1.0	80.3
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	CCTTTAGCGCTGCCCAATAT	50.0	50.0	58.0	58.4	1339-1364 bp	1.0	80.2
ndhF-rpl32_p5	GTTGCCGTCGGAAAAAGAAG	TTTAGCGCTGCCCAATATCC	50.0	50.0	58.0	58.4	1337-1362 bp	1.0	80.2