Hotspot

atpB-rbcL

LSC · 56228-56984 · 757 bp

Back to Deutzia

Pi 0.00346

MarkerSeek score 59.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 11
pi: 0.00346
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AGCTACATCTAGGACCGGAC	GCTTTGAATCCAACACCTGC	55.0	50.0	58.0	57.9	886-912 bp	1.0	82.8
atpB-rbcL_p2	GGACCGGACCAATGATTTGA	GCTTTGAATCCAACACCTGC	50.0	50.0	57.9	57.9	875-901 bp	1.0	82.0
atpB-rbcL_p3	AGGACCGGACCAATGATTTG	GCTTTGAATCCAACACCTGC	50.0	50.0	57.9	57.9	876-902 bp	1.0	81.9
atpB-rbcL_p4	ATCTTGCCTGGAGGAAAAGC	GCTTTGAATCCAACACCTGC	50.0	50.0	58.2	57.9	903-929 bp	1.0	81.6
atpB-rbcL_p5	ACATCTAGGACCGGACCAAT	GCTTTGAATCCAACACCTGC	50.0	50.0	57.8	57.9	882-908 bp	1.0	81.5