Hotspot

trnS-GCU-trnG-UCC

LSC · 9074-9836 · 763 bp

Back to Deutzia

Pi 0.00477

MarkerSeek score 54.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 16
pi: 0.00477
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AATCCAAACCGAAAGACCCC	50.0	50.0	58.0	58.1	874-897 bp	1.0	81.0
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AATCCAAACCGAAAGACCCC	50.0	50.0	58.0	58.1	873-896 bp	1.0	80.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AATCCAAACCGAAAGACCCC	50.0	50.0	58.2	58.1	928-951 bp	1.0	79.7
trnS-GCU-trnG-UCC_p4	AGCAATCCGACGCTTTAGTC	AATCCAAACCGAAAGACCCC	50.0	50.0	58.4	58.1	876-899 bp	1.0	78.1
trnS-GCU-trnG-UCC_p5	CGACGCTTTAGTCCACTCAG	AATCCAAACCGAAAGACCCC	55.0	50.0	58.4	58.1	869-892 bp	1.0	78.0