Hotspot

rnl-trnA-UGC

Genome · 128662-128721 · 60 bp

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Pi 0.0667
MarkerSeek score 83.30
Primer available yes
Label rnl-trnA-UGC

Feature Metadata

label_name
rnl-trnA-UGC
feature_type
igs
region
Genome
length_bp
60
variable_sites
4
pi
0.0667
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rnl-trnA-UGC_p1 CTGAATGCCAAGGTATCCCC CCCTGATAAGGGTGAGGTCT 55.0 55.0 58.0 57.8 224 bp 1.0 79.9
rnl-trnA-UGC_p2 CGTCCTTCATCGCTTCTGAA CCCTGATAAGGGTGAGGTCT 50.0 55.0 58.0 57.8 239 bp 1.0 79.8
rnl-trnA-UGC_p3 TGCCAAGGTATCCCCCATAA CCCTGATAAGGGTGAGGTCT 50.0 55.0 58.1 57.8 219 bp 1.0 79.3
rnl-trnA-UGC_p4 GAGACCTCCGGATCATAGCT CCCTGATAAGGGTGAGGTCT 55.0 55.0 58.1 57.8 295 bp 1.0 79.0
rnl-trnA-UGC_p5 TCATCGCTTCTGAATGCCAA CCCTGATAAGGGTGAGGTCT 45.0 55.0 57.9 57.8 233 bp 1.0 79.0