Hotspot

rpl32-psbY

Genome · 124640-125314 · 675 bp

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Pi 0.167
MarkerSeek score 82.30
Primer available yes
Label rpl32-psbY

Feature Metadata

label_name
rpl32-psbY
feature_type
igs
region
Genome
length_bp
675
variable_sites
91
pi
0.167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-psbY_p1 TGCTTTTGATGTCCGTTTTT TTAGTAGCAGCATCATGGGC 35.0 50.0 54.6 57.7 676-783 bp 1.0 62.7
rpl32-psbY_p2 TTGCTTTTGATGTCCGTTTT TTAGTAGCAGCATCATGGGC 35.0 50.0 54.6 57.7 677-784 bp 1.0 62.7
rpl32-psbY_p3 TTTGCTTTTGATGTCCGTTT TTAGTAGCAGCATCATGGGC 35.0 50.0 54.6 57.7 678-785 bp 1.0 62.7
rpl32-psbY_p4 TTTTGCTTTTGATGTCCGTT TTAGTAGCAGCATCATGGGC 35.0 50.0 54.6 57.7 679-786 bp 1.0 62.7
rpl32-psbY_p5 TTTTTGCTTTTGATGTCCGT TTAGTAGCAGCATCATGGGC 35.0 50.0 54.6 57.7 680-787 bp 1.0 62.7