Hotspot

trnQ-UUG-trnR-ACG

Genome · 87974-88039 · 66 bp

Back to Detonula

Pi 0.108
MarkerSeek score 79.90
Primer available yes
Label trnQ-UUG-trnR-ACG

Feature Metadata

label_name
trnQ-UUG-trnR-ACG
feature_type
igs
region
Genome
length_bp
66
variable_sites
7
pi
0.108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnR-ACG_p1 CAAAGTCCACAGCCTTACCA GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 126-127 bp 1.0 80.9
trnQ-UUG-trnR-ACG_p2 AAAGTCCACAGCCTTACCAC GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 125-126 bp 1.0 80.9
trnQ-UUG-trnR-ACG_p3 GAGTCAAAGTCCACAGCCTT GGGGTTCGAATCCCTTCTTG 50.0 55.0 57.7 58.2 130-131 bp 1.0 80.8
trnQ-UUG-trnR-ACG_p4 GTCCACAGCCTTACCACTTG GGGGTTCGAATCCCTTCTTG 55.0 55.0 58.5 58.2 122-123 bp 1.0 79.4
trnQ-UUG-trnR-ACG_p5 GTGGCAGGATTCGAACCTAC GGGGTTCGAATCCCTTCTTG 55.0 55.0 58.3 58.2 158-159 bp 1.0 79.3