Hotspot

thiS-psb28

Genome · 86939-87465 · 527 bp

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Pi 0.263

MarkerSeek score 79.20

Primer available yes

Label thiS-psb28

Feature Metadata

label_name: thiS-psb28
feature_type: igs
region: Genome
length_bp: 527
variable_sites: 117
pi: 0.263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiS-psb28_p1	TCACAACCCACTGCCTTAAC	CTTTGACTCCGCCATTCGTA	50.0	50.0	58.0	58.0	1299-1341 bp	1.0	91.8
thiS-psb28_p2	TCACAACCCACTGCCTTAAC	TTTGACTCCGCCATTCGTAG	50.0	50.0	58.0	58.0	1298-1340 bp	1.0	91.8
thiS-psb28_p3	TCACAACCCACTGCCTTAAC	AAAAGTGCTGAAGCTTGGGA	50.0	45.0	58.0	57.9	905-948 bp	1.0	91.2
thiS-psb28_p4	GACGACGCGCTTCTTTAATG	CTTTGACTCCGCCATTCGTA	50.0	50.0	57.9	58.0	1684 bp	0.5	59.5
thiS-psb28_p5	GACGACGCGCTTCTTTAATG	TTTGACTCCGCCATTCGTAG	50.0	50.0	57.9	58.0	1683 bp	0.5	59.5