Hotspot

psbA-ycf35

Genome · 83096-83571 · 476 bp

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Pi 0.334
MarkerSeek score 84.70
Primer available yes
Label psbA-ycf35

Feature Metadata

label_name
psbA-ycf35
feature_type
igs
region
Genome
length_bp
476
variable_sites
145
pi
0.334
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-ycf35_p1 GCTCCAGCTGTAAATGCTTA TGGAATCTCTAACTTATTTAATGCA 45.0 28.0 55.8 54.6 573-578 bp 1.0 52.0
psbA-ycf35_p2 GCTCCAGCTGTAAATGCTTAA TGGAATCTCTAACTTATTTAATGCA 42.9 28.0 56.6 54.6 573-578 bp 1.0 52.0
psbA-ycf35_p3 GCTCCAGCTGTAAATGCTTA ATGGAATCTCTAACTTATTTAATGCA 45.0 26.9 55.8 55.1 574-579 bp 1.0 52.0
psbA-ycf35_p4 GCTCCAGCTGTAAATGCTTA TGGAATCTCTAACTTATTTAATGCAT 45.0 26.9 55.8 55.1 573-578 bp 1.0 52.0
psbA-ycf35_p5 GCTCCAGCTGTAAATGCTTA CTTGTTTTCAAATTTGTAAAATGTGAC 45.0 25.9 55.8 55.9 521-526 bp 1.0 52.0