Hotspot

psbY-rpl32

Genome · 73473-74147 · 675 bp

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Pi 0.167
MarkerSeek score 82.30
Primer available yes
Label psbY-rpl32

Feature Metadata

label_name
psbY-rpl32
feature_type
igs
region
Genome
length_bp
675
variable_sites
91
pi
0.167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbY-rpl32_p1 TTAGTAGCAGCATCATGGGC TGCTTTTGATGTCCGTTTTT 50.0 35.0 57.7 54.6 676-783 bp 1.0 62.7
psbY-rpl32_p2 TTAGTAGCAGCATCATGGGC TTGCTTTTGATGTCCGTTTT 50.0 35.0 57.7 54.6 677-784 bp 1.0 62.7
psbY-rpl32_p3 TTAGTAGCAGCATCATGGGC TTTGCTTTTGATGTCCGTTT 50.0 35.0 57.7 54.6 678-785 bp 1.0 62.7
psbY-rpl32_p4 TTAGTAGCAGCATCATGGGC TTTTGCTTTTGATGTCCGTT 50.0 35.0 57.7 54.6 679-786 bp 1.0 62.7
psbY-rpl32_p5 TTAGTAGCAGCATCATGGGC TTTTTGCTTTTGATGTCCGT 50.0 35.0 57.7 54.6 680-787 bp 1.0 62.7