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trnT-UGU-ycf33

Genome · 49716-50214 · 499 bp

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Pi 0.227

MarkerSeek score 81.30

Primer available yes

Label trnT-UGU-ycf33

Feature Metadata

label_name: trnT-UGU-ycf33
feature_type: igs
region: Genome
length_bp: 499
variable_sites: 107
pi: 0.227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-ycf33_p1	TCGAATTGTTTGTGGACGGT	CGACCTTGGGCTTATGAGTC	45.0	55.0	58.0	58.1	1489-1538 bp	1.0	88.8
trnT-UGU-ycf33_p2	TCGAATTGTTTGTGGACGGT	TAGTAGATGGCTCTGGTGGG	45.0	55.0	58.0	57.9	1520-1569 bp	1.0	88.6
trnT-UGU-ycf33_p3	AGAAGCTTGAACGATCGCAT	CGACCTTGGGCTTATGAGTC	45.0	55.0	58.0	58.1	1453 bp	0.5	59.4
trnT-UGU-ycf33_p4	AGAAGCTTGAACGATCGCAT	TAGTAGATGGCTCTGGTGGG	45.0	55.0	58.0	57.9	1484 bp	0.5	59.2
trnT-UGU-ycf33_p5	AGAAGCTTGAACGATCGCAT	ACCTACGACCTTGGGCTTAT	45.0	50.0	58.0	58.1	1458 bp	0.5	58.9