Hotspot

petA-chlI

Genome · 45127-45618 · 492 bp

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Pi 0.316

MarkerSeek score 81.30

Primer available yes

Label petA-chlI

Feature Metadata

label_name: petA-chlI
feature_type: igs
region: Genome
length_bp: 492
variable_sites: 148
pi: 0.316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-chlI_p1	CTGTTGTTGCACCTGATCCT	GCAAAAGCACATGCAGCATA	50.0	45.0	58.1	58.0	1133-1187 bp	1.0	88.8
petA-chlI_p2	CCGTGGTCAAGTTTACCCAA	GCAAAAGCACATGCAGCATA	50.0	45.0	58.0	58.0	1054 bp	0.5	59.8
petA-chlI_p3	TGGTCAAGTTTACCCAACGG	GCAAAAGCACATGCAGCATA	50.0	45.0	58.0	58.0	1051 bp	0.5	59.8
petA-chlI_p4	CCGTGGTCAAGTTTACCCAA	TTGGTATGCACGCAGAGATC	50.0	50.0	58.0	58.1	1320 bp	0.5	59.3
petA-chlI_p5	TGGTCAAGTTTACCCAACGG	TTGGTATGCACGCAGAGATC	50.0	50.0	58.0	58.1	1317 bp	0.5	59.3