Hotspot

psbT-pbf1

Genome · 19064-19089 · 26 bp

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Pi 0.192

MarkerSeek score 82.40

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: Genome
length_bp: 26
variable_sites: 5
pi: 0.192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	TCTTTGCTGTATTCTTCCGTGA	TACTGCTTTTGGACCTGCTT	40.9	45.0	57.7	57.3	134 bp	1.0	59.6
psbT-pbf1_p2	TCTTTGCTGTATTCTTCCGTGA	ACTGCTTTTGGACCTGCTTC	40.9	50.0	57.7	58.7	133 bp	1.0	59.5
psbT-pbf1_p3	TCTTTGCTGTATTCTTCCGTGA	ATACTGCTTTTGGACCTGCT	40.9	45.0	57.7	57.1	135 bp	1.0	57.7
psbT-pbf1_p4	CTTTGCTGTATTCTTCCGTGA	TACTGCTTTTGGACCTGCTT	42.9	45.0	56.3	57.3	133 bp	1.0	56.0
psbT-pbf1_p5	TCTTTGCTGTATTCTTCCGTG	TACTGCTTTTGGACCTGCTT	42.9	45.0	56.3	57.3	134 bp	1.0	56.0