Hotspot

atpB

Genome · 103071-108415 · 5345 bp

Back to Desmodesmus

Pi 0.0806

MarkerSeek score 71.40

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 5345
variable_sites: 425
pi: 0.0806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	ACCTTTTCGAATACTCGCCC	TACCCTTGGACCACCCTTAG	50.0	55.0	58.0	58.0	1538 bp	1.0	87.5
atpB_p2	CGAACTGTACGTGCACCTTA	TACCCTTGGACCACCCTTAG	50.0	55.0	57.9	58.0	1517 bp	1.0	87.3
atpB_p3	TTCAGGCATGGCTGGATTTT	TACCCTTGGACCACCCTTAG	45.0	55.0	58.1	58.0	1268 bp	0.5	59.2
atpB_p4	ACCTTTTCGAATACTCGCCC	TCCTTGTTGCAGGGAATGTC	50.0	50.0	58.0	58.1	972 bp	0.5	59.1
atpB_p5	ACCTTTTCGAATACTCGCCC	TGTGCAGTTTGTGGTTCAGA	50.0	45.0	58.0	57.9	1085 bp	0.5	58.9