Hotspot

ODF51_pgt024

Genome · 121124-121196 · 73 bp

Back to Desmodesmus

Pi 0.0548

MarkerSeek score 73.60

Primer available yes

Label ODF51_pgt024

Feature Metadata

label_name: ODF51_pgt024
feature_type: tRNA
region: Genome
length_bp: 73
variable_sites: 4
pi: 0.0548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ODF51_pgt024_p1	TCTCAAACTCTTTCCAAGGGAC	ACAACTTTGTGAAGTAGAAAAAGGT	45.5	32.0	57.9	57.5	184 bp	1.0	41.7
ODF51_pgt024_p2	TCTCAAACTCTTTCCAAGGGA	ACAACTTTGTGAAGTAGAAAAAGGT	42.9	32.0	56.5	57.5	184 bp	1.0	41.7
ODF51_pgt024_p3	CTCAAACTCTTTCCAAGGGAC	ACAACTTTGTGAAGTAGAAAAAGGT	47.6	32.0	56.5	57.5	183 bp	1.0	41.7
ODF51_pgt024_p4	TCTCAAACTCTTTCCAAGGGAC	ACAACTTTGTGAAGTAGAAAAAGG	45.5	33.3	57.9	56.0	184 bp	1.0	41.7
ODF51_pgt024_p5	TCTCAAACTCTTTCCAAGGGAC	CAACTTTGTGAAGTAGAAAAAGGT	45.5	33.3	57.9	56.0	183 bp	1.0	41.7