Hotspot

ODF51_pgt022-ODF51_pgt023

Genome · 120289-120847 · 559 bp

Back to Desmodesmus

Pi 0.0429
MarkerSeek score 77.20
Primer available yes
Label ODF51_pgt022-ODF51_pgt023

Feature Metadata

label_name
ODF51_pgt022-ODF51_pgt023
feature_type
igs
region
Genome
length_bp
559
variable_sites
23
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ODF51_pgt022-ODF51_pgt023_p1 TTCGAATCCCCGTTTTCTCC GTCGTGGGTTCAAGTCCTAC 50.0 55.0 57.9 57.9 628-646 bp 1.0 84.3
ODF51_pgt022-ODF51_pgt023_p2 TTCGAATCCCCGTTTTCTCC CGTGGGTTCAAGTCCTACAG 50.0 55.0 57.9 57.9 626-644 bp 1.0 84.3
ODF51_pgt022-ODF51_pgt023_p3 TTCGAATCCCCGTTTTCTCC AAACCTGATGTCGTGGGTTC 50.0 50.0 57.9 58.1 637-655 bp 1.0 84.0
ODF51_pgt022-ODF51_pgt023_p4 TTCGAATCCCCGTTTTCTCC TCCAAAACCTGATGTCGTGG 50.0 50.0 57.9 58.1 641-659 bp 1.0 84.0
ODF51_pgt022-ODF51_pgt023_p5 TTCGAATCCCCGTTTTCTCC GGTAGAACGCAGGTTTCCAA 50.0 50.0 57.9 58.1 656-674 bp 1.0 83.8