Hotspot

ODF51_pgt021-psbM

Genome · 111056-111414 · 359 bp

Back to Desmodesmus

Pi 0.0398

MarkerSeek score 73.60

Primer available yes

Label ODF51_pgt021-psbM

Feature Metadata

label_name: ODF51_pgt021-psbM
feature_type: igs
region: Genome
length_bp: 359
variable_sites: 14
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ODF51_pgt021-psbM_p1	TTTGAGCAAGGAGGGATTCG	ACTGCATCAAATGAATCTGCT	50.0	38.1	57.9	56.2	453-459 bp	1.0	60.4
ODF51_pgt021-psbM_p2	CCACATGCTCTAGTCCACTG	ACTGCATCAAATGAATCTGCT	55.0	38.1	57.7	56.2	409-415 bp	1.0	59.3
ODF51_pgt021-psbM_p3	GAGCAAGGAGGGATTCGAAC	ACTGCATCAAATGAATCTGCT	55.0	38.1	58.3	56.2	450-456 bp	1.0	58.6
ODF51_pgt021-psbM_p4	TTTGAGCAAGGAGGGATTCG	AACTGCATCAAATGAATCTGCT	50.0	36.4	57.9	56.9	454-460 bp	1.0	58.2
ODF51_pgt021-psbM_p5	TTTGAGCAAGGAGGGATTCG	ACTGCATCAAATGAATCTGCTT	50.0	36.4	57.9	56.9	453-459 bp	1.0	58.2