Hotspot

psbH-psbK

Genome · 70111-70819 · 709 bp

Back to Desmodesmus

Pi 0.0335

MarkerSeek score 76.80

Primer available yes

Label psbH-psbK

Feature Metadata

label_name: psbH-psbK
feature_type: igs
region: Genome
length_bp: 709
variable_sites: 23
pi: 0.0335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-psbK_p1	ATGGGTGTTTTCATTGGGCT	AAGGTGCATAAGCTTCTGGT	45.0	45.0	58.0	57.1	873-875 bp	1.0	76.9
psbH-psbK_p2	TGGGTGTTTTCATTGGGCTT	AAGGTGCATAAGCTTCTGGT	45.0	45.0	58.2	57.1	872-874 bp	1.0	75.5
psbH-psbK_p3	ATGGGTGTTTTCATTGGGCT	AAAGGTGCATAAGCTTCTGGT	45.0	42.9	58.0	57.8	874-876 bp	1.0	74.7
psbH-psbK_p4	TGGGTGTTTTCATTGGGCTT	AAAGGTGCATAAGCTTCTGGT	45.0	42.9	58.2	57.8	873-875 bp	1.0	73.3
psbH-psbK_p5	ATGGGTGTTTTCATTGGGCT	ACGATAGGATCAAAAGGTGCA	45.0	42.9	58.0	57.4	886-888 bp	1.0	70.9