Hotspot

ODF51_pgt004-atpE

Genome · 53900-54076 · 177 bp

Back to Desmodesmus

Pi 0.0422
MarkerSeek score 80.90
Primer available yes
Label ODF51_pgt004-atpE

Feature Metadata

label_name
ODF51_pgt004-atpE
feature_type
igs
region
Genome
length_bp
177
variable_sites
7
pi
0.0422
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ODF51_pgt004-atpE_p1 GTAAAGCCACGCTGGTTTTC GCACGCTTTCAAGTTGTGAA 50.0 45.0 58.0 57.8 263-274 bp 1.0 83.5
ODF51_pgt004-atpE_p2 GAAAGGGAGGGATTCGAACC GCACGCTTTCAAGTTGTGAA 55.0 45.0 58.0 57.8 293-304 bp 1.0 83.0
ODF51_pgt004-atpE_p3 GGAAAGGGAGGGATTCGAAC GCACGCTTTCAAGTTGTGAA 55.0 45.0 58.0 57.8 294-305 bp 1.0 83.0
ODF51_pgt004-atpE_p4 GAAAGGGAGGGATTCGAACC AAGCACGCTTTCAAGTTGTG 55.0 45.0 58.0 57.8 295-306 bp 1.0 82.9
ODF51_pgt004-atpE_p5 GGAAAGGGAGGGATTCGAAC AAGCACGCTTTCAAGTTGTG 55.0 45.0 58.0 57.8 296-307 bp 1.0 82.9