Hotspot

rpoBb-rpoBa

Genome · 33069-33758 · 690 bp

Back to Desmodesmus

Pi 0.0579

MarkerSeek score 78.10

Primer available yes

Label rpoBb-rpoBa

Feature Metadata

label_name: rpoBb-rpoBa
feature_type: igs
region: Genome
length_bp: 690
variable_sites: 39
pi: 0.0579
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb-rpoBa_p1	AGAGCTGACTTTCTGGGACT	ACAGGTTTTGAAGCACGAGT	50.0	45.0	58.0	58.0	1402-1418 bp	1.0	82.9
rpoBb-rpoBa_p2	AGAGCTGACTTTCTGGGACT	TGATTCAGGGCATGCTGTTT	50.0	45.0	58.0	58.1	1376-1392 bp	1.0	82.6
rpoBb-rpoBa_p3	TGCTCTGCAGAAAGGAAGTG	CACAAACGACGCTTGACTTC	50.0	50.0	58.1	58.0	1417-1433 bp	1.0	82.5
rpoBb-rpoBa_p4	AGAGCTGACTTTCTGGGACT	CACAAACGACGCTTGACTTC	50.0	50.0	58.0	58.0	1936-1952 bp	1.0	82.4
rpoBb-rpoBa_p5	CTTTCACAGAAGTGCCTCGT	CACAAACGACGCTTGACTTC	50.0	50.0	58.1	58.0	1984-2000 bp	1.0	81.5