Hotspot

trnG-GCC

LSC · 62353-62423 · 71 bp

Back to Desmidorchis

Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label trnG-GCC

Feature Metadata

label_name: trnG-GCC
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC_p1	AGGAGAGTCTTTGGTCTGGG	ACTACACGATCACCGACAAC	55.0	50.0	58.3	57.7	263-265 bp	1.0	75.4
trnG-GCC_p2	GGAGAGTCTTTGGTCTGGGA	ACTACACGATCACCGACAAC	55.0	50.0	58.4	57.7	262-264 bp	1.0	75.3
trnG-GCC_p3	AGAGTCTTTGGTCTGGGACT	ACTACACGATCACCGACAAC	50.0	50.0	57.6	57.7	260-262 bp	1.0	74.9
trnG-GCC_p4	AGGAGAGTCTTTGGTCTGGG	GGGAGGCTATAGGAAAGGGA	55.0	55.0	58.3	57.6	301-303 bp	1.0	74.7
trnG-GCC_p5	GGAGAGTCTTTGGTCTGGGA	GGGAGGCTATAGGAAAGGGA	55.0	55.0	58.4	57.6	300-302 bp	1.0	74.6