Hotspot

rps16

LSC · 29724-30818 · 1095 bp

Back to Desmidorchis

Pi 0.00183

MarkerSeek score 59.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1095
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGGGAAAAGGAAAAAGGGG	CTCCGACGTCTACAAAGCAA	50.0	50.0	58.3	57.9	1268 bp	1.0	77.4
rps16_p2	ATATACAAGCAACTGCCCCC	CTCCGACGTCTACAAAGCAA	50.0	50.0	57.6	57.9	1217-1218 bp	1.0	76.8
rps16_p3	ACGGGAAAAGGAAAAAGGGG	GACTCCGACGTCTACAAAGC	50.0	55.0	58.3	58.4	1270 bp	1.0	74.8
rps16_p4	ATATACAAGCAACTGCCCCC	GACTCCGACGTCTACAAAGC	50.0	55.0	57.6	58.4	1219-1220 bp	1.0	74.2
rps16_p5	ACGAGTAGAACGGGAAAAGG	CTCCGACGTCTACAAAGCAA	50.0	50.0	57.3	57.9	1277 bp	1.0	73.8