Hotspot

petD

LSC · 102922-104254 · 1333 bp

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Pi 0

MarkerSeek score 62.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1333
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGGAGTTGAGGGGAATTGTC	TCCCTAGATACACACGTCGT	55.0	50.0	57.9	57.6	1352-1407 bp	1.0	78.9
petD_p2	AGGGAGTTGAGGGGAATTGT	TCCCTAGATACACACGTCGT	50.0	50.0	58.2	57.6	1353-1408 bp	1.0	78.0
petD_p3	GGGAGTTGAGGGGAATTGTC	TTCCCTAGATACACACGTCGT	55.0	47.6	57.9	58.3	1353-1408 bp	1.0	71.9
petD_p4	TAGGGAGTTGAGGGGAATTGT	TCCCTAGATACACACGTCGT	47.6	50.0	58.1	57.6	1354-1409 bp	1.0	71.4
petD_p5	AGGGAGTTGAGGGGAATTGT	TTCCCTAGATACACACGTCGT	50.0	47.6	58.2	58.3	1354-1409 bp	1.0	71.0