Hotspot

matK

LSC · 26616-28172 · 1557 bp

Back to Desmidorchis

Pi 0.00257

MarkerSeek score 60.10

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1557
variable_sites: 4
pi: 0.00257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGTTACACCAACATTTCAGA	AAGTTTACCCGTTTCCGAGG	35.0	50.0	52.6	57.8	1760 bp	1.0	40.2
matK_p2	TGTTACACCAACATTTCAGA	TTTGCTAACCCCAAAACCGT	35.0	45.0	52.6	58.2	1689 bp	1.0	40.2
matK_p3	TGTTACACCAACATTTCAGAA	AAGTTTACCCGTTTCCGAGG	33.3	50.0	53.6	57.8	1760 bp	1.0	40.2
matK_p4	TGTTACACCAACATTTCAGAA	TTTGCTAACCCCAAAACCGT	33.3	45.0	53.6	58.2	1689 bp	1.0	40.2
matK_p5	TGTTACACCAACATTTCAGA	ACCCGTTTCCGAGGTATCTA	35.0	50.0	52.6	57.6	1754 bp	1.0	40.2