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psbE-petL

LSC · 90967-92101 · 1135 bp

Back to Desmidorchis

Pi 0.000881

MarkerSeek score 59.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 1
pi: 0.000881
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCA	45.0	45.0	58.0	56.9	1293 bp	1.0	70.8
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.0	56.9	1292 bp	1.0	70.8
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1282 bp	1.0	70.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1281 bp	1.0	70.5
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1204 bp	1.0	69.7