Hotspot

psaA-ycf3

LSC · 67777-68746 · 970 bp

Back to Desmidorchis

Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCTGCCGAGTATTGG	50.0	50.0	58.1	58.0	1167 bp	1.0	79.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	1148 bp	1.0	78.8
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	CGATCAAGCTGCCGAGTATT	50.0	50.0	58.1	58.1	1169 bp	1.0	78.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	1150 bp	1.0	78.0
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	1096 bp	1.0	78.0