Hotspot

psbZ-trnG-GCC

LSC · 62021-62352 · 332 bp

Back to Desmidorchis

Pi 0

MarkerSeek score 42.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GTGCCCGTTGTATTTGCTTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	768-782 bp	1.0	81.2
psbZ-trnG-GCC_p2	GTGCCCGTTGTATTTGCTTC	TTCATCGACGTTGTTTTGCG	50.0	45.0	58.0	58.0	1074-1088 bp	1.0	80.8
psbZ-trnG-GCC_p3	ATTAGCGTGCCCGTTGTATT	TTCATCGACGTTGTTTTGCG	45.0	45.0	58.0	58.0	1080-1094 bp	1.0	80.7
psbZ-trnG-GCC_p4	CAACCACTCAGCCATCTCTC	GGGTAGAGCAGTTTGGTAGC	55.0	55.0	58.0	58.0	1174-1188 bp	1.0	80.7
psbZ-trnG-GCC_p5	CAACCACTCAGCCATCTCTC	TTCATCGACGTTGTTTTGCG	55.0	45.0	58.0	58.0	1480-1494 bp	1.0	80.6