Hotspot

trnE-UUC-trnT-GGU

LSC · 56422-57444 · 1023 bp

Back to Desmidorchis

Pi 0.000978

MarkerSeek score 59.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1023
variable_sites: 1
pi: 0.000978
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	ACCGGAGTTTTATGGAAGCC	50.0	50.0	57.9	57.9	1143 bp	1.0	77.9
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	ACCGGAGTTTTATGGAAGCC	50.0	50.0	57.9	57.9	1141 bp	1.0	77.9
trnE-UUC-trnT-GGU_p3	CCTTTCCTAGTCCCCTACCC	ACCGGAGTTTTATGGAAGCC	60.0	50.0	58.2	57.9	1199 bp	1.0	77.5
trnE-UUC-trnT-GGU_p4	CTTTCCTAGTCCCCTACCCC	ACCGGAGTTTTATGGAAGCC	60.0	50.0	58.2	57.9	1198 bp	1.0	77.5
trnE-UUC-trnT-GGU_p5	GATGTCCTGAACCGCTAGAC	ACCGGAGTTTTATGGAAGCC	55.0	50.0	57.8	57.9	1140 bp	1.0	77.4