Hotspot

rpoB-trnC-GCA

LSC · 51767-53001 · 1235 bp

Back to Desmidorchis

Pi 0.00162

MarkerSeek score 59.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 2
pi: 0.00162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1323 bp	1.0	78.7
rpoB-trnC-GCA_p2	GCAAACCCCGATCAATGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1391 bp	1.0	77.2
rpoB-trnC-GCA_p3	ATTCCCTCATTTGCATCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1305 bp	1.0	76.5
rpoB-trnC-GCA_p4	GCAAACCCCGATCAATGAAC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1373 bp	1.0	75.0
rpoB-trnC-GCA_p5	ATTCCCTCATTTGCATCCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.9	58.8	1342 bp	1.0	73.3